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cellbind t75 flask  (Millipore)


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    Millipore cellbind t75 flask
    Cellbind T75 Flask, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellbind+t75+flask/cellbind+t75+flask/pm37907765-70-9-12
    Average 90 stars, based on 1 article reviews
    cellbind t75 flask - by Bioz Stars, 2026-10
    90/100 stars

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    Suspension:

    Article Title: Ascorbic Acid 2-Phosphate Releasing Supercritically Foamed Porous Poly-L-Lactide-Co-ε-Caprolactone Scaffold Enhances the Collagen Production of Human Vaginal Stromal Cells: A New Approach for Vaginal Tissue Engineering.
    Article Snippet: .. The suspension was filtered, centrifuged, and plated in a CellBind T75 flask (Sigma-Aldrich, St. Louis, MO, USA) with EpiLifeTM medium (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% EpiLifeTM defined growth supplement (EDGS; Invitrogen), 0.1% CaCl2 (Invitrogen) and 0.35% antibiotics (100 U/ml penicillin and 0.1 mg/ml streptomycin [Lonza, BioWhittaker, Verviers, Belgium]). .. After primary culturing, the EC and SC lines were separated, and the cells were treated with TrypLE Select (Gibco, Thermo Fisher Scientific).

    Article Title: Promoting cell proliferation and collagen production with ascorbic acid 2-phosphate-releasing poly(l-lactide-co-ε-caprolactone) membranes for treating pelvic organ prolapse
    Article Snippet: Briefly, the tissue was cut into small pieces and incubated in a solution containing 1.5 mg/ml collagenase Type I (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA) and 4 mg/ml dispase (Invitrogen, Thermo Fisher Scientific) in a water bath. .. The suspension was filtered, centrifuged and plated to CellBind T75 flask (Sigma-Aldrich, St Louis, MO, USA) with EpiLife medium (Invitrogen) supplemented with 1% EpiLife defined growth supplement (EDGS; Invitrogen), 0.1% of CaCl 2 (Invitrogen) and 0.35% of antibiotics (100 U/ml penicillin and 0.1 mg/ml streptomycin; Lonza, BioWhittaker, Verviers, Belgium). .. After primary culturing, the hVFs were separated from the epithelial cell line by treating the cells with TrypLE Select (Gibco, Thermo Fisher Scientific) and the loosened cells were passaged to T75 flasks (Nunc, Thermo Fisher Scientific) in DMEM/F12 (Thermo Fisher Scientific) supplemented with 5% human serum (HS; Biowest, Nuaillé, France), 1% GlutaMAX (Life Technologies) and 1% of antibiotics (100 U/ml penicillin and 0.1 mg/ml streptomycin; Lonza), and cultured in a humidified atmosphere with 5% of CO 2 .

    Article Title: Ascorbic Acid 2-Phosphate Releasing Supercritically Foamed Porous Poly-L-Lactide-Co-ε-Caprolactone Scaffold Enhances the Collagen Production of Human Vaginal Stromal Cells: A New Approach for Vaginal Tissue Engineering
    Article Snippet: .. The suspension was filtered, centrifuged, and plated in a CellBind T75 flask (Sigma-Aldrich, St. Louis, MO, USA) with EpiLifeTM medium (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% EpiLifeTM defined growth supplement (EDGS; Invitrogen), 0.1% CaCl2 (Invitrogen) and 0.35% antibiotics (100 U/ml penicillin and 0.1 mg/ml streptomycin [Lonza, BioWhittaker, Verviers, Belgium]). .. After primary culturing, the EC and SC lines were separated, and the cells were treated with TrypLE Select (Gibco, Thermo Fisher Scientific).



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    cellbind t75 flask  (Millipore)
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    Schematic representation of experimental designs. All experimental designs commenced with PDL 13 seeded at a density of 3000 cells/cm 2 on Day minus 3 (not shown). (A) Three‐day experiments assessed, in 96‐well plates, the ability of mitomycin C (MMC), sodium arsenite (iAs), or diethanolamine (DEA) to induce micronuclei (MN), which included a 24‐h seeding recovery phase, followed by a 48‐h exposure to media containing test chemicals. (B) Ten‐day experiments investigated the effects of cellular aging and of iAs exposure. Following a 24‐h recovery after seeding, the cells were cultured in T75 flasks for 6 days with increasing concentrations of iAs or vehicle control. The cells were then subjected to the same 3‐day protocol without or with MMC. (C) Short‐duration gene expression experiments followed the same protocol as the standard micronucleus experiment, with RNA extraction occurring at the time that MN would be measured. (D) The longer duration gene expression experiments were conducted over 30 days and involved multiple rounds of cell exposures to iAs or vehicle control.

    Journal: Journal of Applied Toxicology

    Article Title: Impacts of Inorganic Arsenic Exposure on Genetic Stability of Human Mesenchymal Stromal Cells

    doi: 10.1002/jat.4785

    Figure Lengend Snippet: Schematic representation of experimental designs. All experimental designs commenced with PDL 13 seeded at a density of 3000 cells/cm 2 on Day minus 3 (not shown). (A) Three‐day experiments assessed, in 96‐well plates, the ability of mitomycin C (MMC), sodium arsenite (iAs), or diethanolamine (DEA) to induce micronuclei (MN), which included a 24‐h seeding recovery phase, followed by a 48‐h exposure to media containing test chemicals. (B) Ten‐day experiments investigated the effects of cellular aging and of iAs exposure. Following a 24‐h recovery after seeding, the cells were cultured in T75 flasks for 6 days with increasing concentrations of iAs or vehicle control. The cells were then subjected to the same 3‐day protocol without or with MMC. (C) Short‐duration gene expression experiments followed the same protocol as the standard micronucleus experiment, with RNA extraction occurring at the time that MN would be measured. (D) The longer duration gene expression experiments were conducted over 30 days and involved multiple rounds of cell exposures to iAs or vehicle control.

    Article Snippet: The concentration of live cells was then determined with an automated cell counter and then seeded at a density of 3333 viable cells/cm 2 in CellBIND T75 flasks (Corning) with 10‐mL XR media.

    Techniques: Cell Culture, Control, Gene Expression, RNA Extraction